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rabbit polyclonal proteintech 10478 2 ap  (Proteintech)


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    Proteintech rabbit polyclonal proteintech 10478 2 ap
    Rabbit Polyclonal Proteintech 10478 2 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal proteintech 10478 2 ap/product/Proteintech
    Average 95 stars, based on 48 article reviews
    rabbit polyclonal proteintech 10478 2 ap - by Bioz Stars, 2026-02
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    rabbit polyclonal proteintech 10478 2 ap  (Proteintech)
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    Figure 4. Decreased activation of METTL3-deficient BM neutrophils is regulated by targeting TLR4 signaling (A) m6A peaks enriched in the 30 UTR of CXCR2 mRNAs in BM neutrophils profiled using MeRIP-seq. (B) CXCR2 and CXCR4 levels were analyzed by western blotting in METTL3 cKO neutrophils and METTL3 WT neutrophils. (C) CXCR2 and CXCR4 mRNA levels were analyzed by qPCR in METTL3 cKO neutrophils and METTL3 WT neutrophils. Data are represented as mean SEM. (D) MeRIP-seq data showed the GO enrichment analysis in the biological process category of transcripts bearing m6A modification only in BM neutrophils from METTL3 cKO and METTL3 WT mice. (E and F) TLR4 membrane expression in BM neutrophils in METTL3 cKO mice and METTL3 WT mice with or without LPS treatment (F, n = 5 mice per group). (G) Relative expression of TLR4, Tirap, <t>Irak1,</t> Traf6, and Irak4 in METTL3 cKO neutrophils and METTL3 WT neutrophils measured by real-time qPCR (5 biological replicates, t test). (H) Activation of the TLR4 signaling pathway in METTL3 cKO and METTL3 WT BM neutrophils upon LPS stimulation was analyzed by western blotting of p65, JNK, p38, ERK, TLR4, Tirap, and Irak1. Statistical significance was set at *p < 0.05, **p < 0.01, ***p < 0.001. Data are represented as mean ± SEM.
    Irak1 Polyclonal Antibody Proteintech 10478 2 Ap Tirap D6m9z Rabbit Mab, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Figure 4. Decreased activation of METTL3-deficient BM neutrophils is regulated by targeting TLR4 signaling (A) m6A peaks enriched in the 30 UTR of CXCR2 mRNAs in BM neutrophils profiled using MeRIP-seq. (B) CXCR2 and CXCR4 levels were analyzed by western blotting in METTL3 cKO neutrophils and METTL3 WT neutrophils. (C) CXCR2 and CXCR4 mRNA levels were analyzed by qPCR in METTL3 cKO neutrophils and METTL3 WT neutrophils. Data are represented as mean SEM. (D) MeRIP-seq data showed the GO enrichment analysis in the biological process category of transcripts bearing m6A modification only in BM neutrophils from METTL3 cKO and METTL3 WT mice. (E and F) TLR4 membrane expression in BM neutrophils in METTL3 cKO mice and METTL3 WT mice with or without LPS treatment (F, n = 5 mice per group). (G) Relative expression of TLR4, Tirap, Irak1, Traf6, and Irak4 in METTL3 cKO neutrophils and METTL3 WT neutrophils measured by real-time qPCR (5 biological replicates, t test). (H) Activation of the TLR4 signaling pathway in METTL3 cKO and METTL3 WT BM neutrophils upon LPS stimulation was analyzed by western blotting of p65, JNK, p38, ERK, TLR4, Tirap, and Irak1. Statistical significance was set at *p < 0.05, **p < 0.01, ***p < 0.001. Data are represented as mean ± SEM.

    Journal: Cell reports

    Article Title: METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling.

    doi: 10.1016/j.celrep.2023.112259

    Figure Lengend Snippet: Figure 4. Decreased activation of METTL3-deficient BM neutrophils is regulated by targeting TLR4 signaling (A) m6A peaks enriched in the 30 UTR of CXCR2 mRNAs in BM neutrophils profiled using MeRIP-seq. (B) CXCR2 and CXCR4 levels were analyzed by western blotting in METTL3 cKO neutrophils and METTL3 WT neutrophils. (C) CXCR2 and CXCR4 mRNA levels were analyzed by qPCR in METTL3 cKO neutrophils and METTL3 WT neutrophils. Data are represented as mean SEM. (D) MeRIP-seq data showed the GO enrichment analysis in the biological process category of transcripts bearing m6A modification only in BM neutrophils from METTL3 cKO and METTL3 WT mice. (E and F) TLR4 membrane expression in BM neutrophils in METTL3 cKO mice and METTL3 WT mice with or without LPS treatment (F, n = 5 mice per group). (G) Relative expression of TLR4, Tirap, Irak1, Traf6, and Irak4 in METTL3 cKO neutrophils and METTL3 WT neutrophils measured by real-time qPCR (5 biological replicates, t test). (H) Activation of the TLR4 signaling pathway in METTL3 cKO and METTL3 WT BM neutrophils upon LPS stimulation was analyzed by western blotting of p65, JNK, p38, ERK, TLR4, Tirap, and Irak1. Statistical significance was set at *p < 0.05, **p < 0.01, ***p < 0.001. Data are represented as mean ± SEM.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD90/THY-1 ANTIBODY, PE CONJUG Thermo MA5-17749 Ms NK-1.1 PE PK136 BD Pharmingen 557391 Ms CD45R/B220 PE RA3-6B2 BD Pharmingen 553089 Ms CD117 BV421 2B8 BD Pharmingen 562609 Ms Siglec-F PerCP-Cy5.5 E50-2440 BD Pharmingen 565526 ANTI-M CD115 AFS98 PERCP-EF710 Thermo 46-1152-82 Ms Ly-6G Ly-6C BV510 RB6-8C5 BD Pharmingen 563040 CD11b FITC M1/70 BD Pharmingen 557396 PE/DazzleTM 594 anti-mouse CD184 (CXCR4) Biolegend 146514 BV786 Rat Anti-Mouse CD182 (CXCR2) BD Pharmingen 747811 PE/Cy7 anti-mouse CD182 (CXCR2) Biolegend 149316 Fixable Viability Stain 780 BD Pharmingen 565388 CD34-APC Biolegend 128612 CD16/CD32-Percp-Cy5.5 Biolegend 101324 Mouse CXCR1/IL-8RA PE-conjugated Antibody R&D FAB8628P-100 CD45 Monoclonal Antibody (30-F11), PE, eBioscienceTM Invitrogen 12-0451-82 PerCP/Cyanine5.5 anti-mouse CD45 Antibody Biolegend 103132 PE Rat Anti-Mouse TNF BD 554419 APC Rat Anti-Mouse IL-6 BD 561367 Anti-N6-methyladenosine (m6A) antibody abcam ab232905 Antibody-METTL3 antibody(EPR18810 abcam AB195352 b-Actin (8H10D10) Mouse mAb #3700 CST #3700 Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb #3675 CST #3675 Myosin Light Chain 2 Antibody #3672 CST #3672 PI3 Kinase p110g (D55D5) Rabbit mAb #5405 CST #5405 PI3 Kinase p110a (C73F8) Rabbit mAb #4249 CST #4249 IRAK1 Polyclonal Antibody proteintech 10478-2-AP TIRAP (D6M9Z) Rabbit mAb (Mouse Specific) #13077 CST #13077 CXCR2 Polyclonal Antibody proteintech 20634-1-AP Anti-CXCR4 antibody [EPUMBR3] (ab181020) abcam ab181020 CXCR1 Polyclonal Antibody proteintech 55450-1-AP CXCR1 Polyclonal Antibody proteintech 55450-1-AP TLR4 Monoclonal Antibody proteintech 66350-1-Ig Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP Rabbit mAb #4511 CST #4511 Anti-MEK1 + MEK2 antibody [EPR16667] (ab178876) abcam ab178876 Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564) abcam ab278564 TLR4 antibody (76B357.1): sc-52962 Santa Cruz Biotechnology 52962 GRK2 antibody (C-9): sc-13143 Santa Cruz Biotechnology sc-13143 NF-kB p65 Monoclonal antibody proteintech Cat No. 66535-1-Ig Anti-NF-kB p65 antibody [E379] (ab32536) abcam ab32536 Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) abcam ab76302 Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668 CST 4668T (Continued on next page) Cell Reports 42, 112259, March 28, 2023 15

    Techniques: Activation Assay, Western Blot, Membrane, Expressing